• Internal archiving tool will help you to keep your important semen analysis and patient data save as well as to make archives with older information to free your storage space.
• There are many security tools and procedures to ensure the integrity of your sperm analyzer data. Database records are deleted to internal recycle bin and removed permanently only after you empty your bin. Images and videos are deleted to Windows recycle bin and can be restored. To prevent unwanted changes in the database records, all the records are locked for editing until you press a special button.
• To avoid unwanted changes by unauthorized personnel, you can enable PIN mode. The PIN mode will disable the following options until you enter correct PIN: edit database structure (add/delete fields), edit print templates, making changes in the autotext box, deleting database filters, changing default calibrations.
• Database structure is implemented as a tree of folders with separate technical files, images, videos, 3rd-party files for every record. This provides the advantage of loading only one record at a time, which makes your work with a large database fast and effective. Furthermore, storage of data in multiple files will protect you in case your PC is infected with a virus: if the virus destroys some files, you will lose only several records and in no case the whole database.
• The database is written in XML Unicode which provides wide options of integration of your data in 3rd-party databases as well as the ability to use your local language to fill in the database fields.
• Database structure can be easily adjusted by the user through a simple and visually clear interface. Add fields for storing text data (also multiline fields for large amounts of text, drop-down lists with predefined values or automatically generated lists for fast selection of repeating words and phrases), numerical data (e.g. parameter values), use special date tool with calendar. Move single fields or large groups of fields to organize your working space. Rename fields as you need, add any number of fields to store additional sperm analysis data along with automatically calculated parameters.
• Use fast search option to find required data in the fields or to retrieve images, video, documents.
• Apply extended filters to make selection of records according to any number of parameters (e.g. records created by certain doctor at certain time period having certain diagnosis etc.) Easy filters can be created fast, complicated multiparametric filters can be saved to filter list and reapplied any time.
• Numeric fields can be set up to count records in the database automatically according to required parameters (e.g. you can keep track of the number of records with certain diagnosis in your database etc.)
• Special autotext box allows you to make an extended tree of predefined text blocks (nomenclature, ready-made phrases describing the diagnosis, prescriptions etc.) Making an advanced tree will let you fill in the patient record with a few clicks without the need of typing long phrases again and again which saves your time and helps to avoid misprints in the record.
• Automatic diagnosis. Autotext box also contains special tool which combines database filters and autoinsertion of text blocks to required text fields. Create a filter describing a diagnosis (if a number of parameters meets normal limits) and select this filter as condition of insertion of the diagnosis into corresponding field. This will allow you to provide exact automatic diagnosis with one click without the need to look through a number of parameters and compare them to normal values.
• Multilanguage support. If your clinic provides consulting in several languages, using autotext insertion based on filters, you can add diagnosis and other text blocks in as many languages as you need. Add a field marking required language and translate your text with one button.
• Database can be displayed in the table mode which allows you to keep track of all the statistics and make your own local reference values of all the measured parameters.

• Dose volume - volume of the semen container to be used (straws, tubes etc.)
• Number of sperm per dose - required number of sperms in a dose.
• By default, the calculation is based on Motility routine results: percentage of progressively moving sperms (A+B%).
• Consider vitality - calculation with respect to percentage of live spermatozoa in the sample if sperm vitality has been tested.
• Consider morphology - calculation with respect to percentage of sperms with normal morphology if sperm morphology has been tested.

• 64 bit Windows 10 (Windows 7, 8 supported but no recommended).
• If possible, avoid laptops since their components provide less performance, use desktop PCs.
• Intel chipsets (no AMD chipsets), chipsets with Z mark recommended, Z270 and higher recommended. Check currently available processors and chipsets on Intel web page
• Intel i5, i7, i9, Xeon processors, 7th generation and higher, Intel HD Graphics 630 and higher.
• Minimum 8 Gb operating memory, DDR4 recommended.

• Intel Core i7-7700HQ
• Intel Core i7-7820HQ
• Intel Core i7-7820HK
• Intel Core i7-7920HQ

• Trinocular upright transmitted light laboratory grade microscope to mount camera on it. Beam splitter 100/0 (all light should be transferred to camera).
• Camera adapter corresponding with image sensor size. We recommend 1/2"-1/1.8" sensor with 1.0x adapter. Larger sensors might be used while applying Region of Interest in camera drivers in motility mode since with 10x objective the sperms might appear too small for comfortable quality control.
• You can choose bright field or phase contrast. MMC Sperm software is able to detect both dark objects on light background and vice versa. Negative phase contrast provides the best results! Our MMC-SK counting chamber due to its transparent glass base unit works very well with phase contrast even on condensers with standard working distance.
• 10x lens for motility. 10x objective provides required focus depth. 20x lenses usually can not get all sperms in focus even with 10 micron deep chambers which disturbs sperm detection. If you want to use additional 20x lens for manual sperm count on counting chambers like the one from Makler, a lens with long working distance should be used. In case of disposable chambers like Leja, there is no need in long WD 20x lens. We recommend using negative phase contrast: sperm heads are covered with light accurately, the background is dark, a lot of debris is being eliminated. Positive phase contrast and bright field are available but have their downsides. Positive phase contrast does not cover the whole sperm head which does not allow us to use head form parameters to differentiate sperms from debris. Furthermore, positive phase contrast might provide halos around sperm heads which can be detected as additional sperms by the software. Bright field makes it harder to differentiate between sperms and debris.
• 100x oil bright field lens for morphology.
• 40x bright field lens for vitality and DNA fragmentation analysis.
• Light source: 30W halogen (or higher). Equivalent LED is highly recommended since halogen lamps might provide flickering at 60 fps if microscope power unit is not stabilized well enough.
• Stage micrometer - calibration scale or graticule. A transparent slide with 1mm scale with 100 divisions 0.01mm.
• Details on microscope specification for MMC Sperm.

• We test cameras for suitability, performance and reliability. Cameras that we recommend keep fine balance between image sensor quality, speed and cost-effectiveness.
• For sperm motility, image clips with 1.3 M resolution and higher at minimum of 60 fps are analyzed directly from PC memory without recording to disk. This provides the maximum performance on minimum of PC hardware. Using third party cameras, you will have to record clips to disk which decreases the analysis speed.
• The camera has got the ability to record all the settings you have applied for motility, morphology, vitality and DNA fragmentation as well as load them automatically when corresponding analysis starts.
• Our direct drivers provide flexible set up of all essential camera parameters which allows you to solve both standard routine and complicated research tasks.
• Details on choosing camera, PC and microscope for MMC Sperm.

Making smears for staining for sperm morphology. (a) Feathering method for undiluted semen. The semen drop (S) spreads along the back edge of the angled slide and is pulled forward over the flat slide to form the smear. (b) Pipette method for washed samples. A drop of the suspension (S) is spread over the surface of the slide by the horizontally held pipette (P).

• NaCl, 0,9% (w/v): dissolve 0,9 g NaCl in 100 ml purified water.
• Eosin Y, 0.5% (w/v): dissolve 0.5 g of eosin Y (colour index 45380) in 100 ml of 0.9% NaCl.
• Some commercially available eosin solutions are hypotonic aqueous solutions that will stress the spermatozoa and can yield false-positive results. To use such a solution, add 0.9 g of NaCl to 100 ml of solution to raise the osmolality.

• Mix the sample well but avoid bubbles.
• Mix 5 microliter of semen with 5 microliter of eosin solution.
• Prepare a smear as described above for sperm morphology smears.